Course: Design of Primers (initiators)
Duration: 4 classes 4:00 p.m. to 6:00 p.m.
Masterclass (webinar) GRATIS: 23 de enero, 2025
Registro: https://www.pharbiois.com/contacto
Course start: February 7-10, 2022
Validez oficial/curricular de la red SEP-CONOCER EC0301
Mode: Online: ZOOM
Spanish Language
about the course
In this course the student will learn to design their primers or initiators, in addition to reviewing concepts about molecular weights in gels as well as the use of bioinformatics tools for design and analysis.
TEMARY
DAY 1
A PDF MANUAL OF PCR
BIOCHEMISTRY OF NUCLEIC ACIDS
CENTRAL DOGMA OF MOLECULAR BIOLOGY
ELECTROPHORESIS IN AGAROSE AND POLYACRYLAMIDE GELS
DAY 2.
GENERAL PRINCIPLES OF THE POLYMERASE CHAIN REACTION
GENERAL PRINCIPLES OF THE DESIGN OF PRIMERS OR INCIATORS
INITIATOR MANUAL DESIGN
PRIMERS DESIGN TO AMPLIFY DNA
VIRTUAL PRACTICE WITH THE NCBI AND BLAST
DAY 3
THE COMPONENTS OF THE PCR
PRACTICAL PRIMER DESIGN USING THE BETA GLOBIN, ACTIN AND ABCB5 GENE
PRIMERS DESIGN TO AMPLIFY cDNA and RETRO TRANSCRIPT
PRIMERS DESIGN WITH PROGRAM
DAY 4
WORKSHOP WITH RESULTS
ANALYSIS AND REPORT OF PCR AMPLIFICATIONS OR AMPLICONS
GENOMIC DNA AMPLIFICATION
AMPLIFICATION TYPE RT-PCR
REAL TIME PCR
Do you want to know more about the agenda? Download the PDF version